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Please note that all translations are automatically generated. Click here for the English version.
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01:17 min
March 15th, 2024
DOI :
10.3791/201643-v
Transcript
首先,在锥形管中获得富集的活 iPSC 衍生细胞悬液,并在 4 摄氏度下以 460 g 离心 5 分钟。弃去上清液后,加入 100 微升新鲜制备的 0.5X 冷裂解缓冲液。使用 P100 上下移液 10 次以混合细胞并将试管在冰上孵育 3 分钟。
接下来,向裂解物中加入 500 微升冷冻洗涤缓冲液,离心管,弃去上清液。重复洗涤后,将沉淀重悬于 120 微升冷冻稀释的核缓冲液中。使用 40 微米细胞过滤器,过滤细胞悬浮液并向滤液中加入 0.4% 台盼蓝。
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