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01:42 min
January 12th, 2024
DOI :
10.3791/201667-v
Transcript
首先,将安乐死的小鼠放在软木板上,腹侧朝上。用镊子提起腹部中央的剃光皮肤,然后用一把无菌剪刀切开一个切口。切开皮肤,将其与腹膜分离。
然后提起腹膜层并切割它。用一套新的无菌镊子和剪刀,轻轻握住、提起和剪开位于睾丸上方的整个附睾脂肪组织。将切除的脂肪放入装有冰上基础培养基的 50 毫升无菌锥形管中。
接下来,用PBS清洗附睾组织。用无菌镊子将附睾脂肪组织转移到装有 15 至 20 毫升消化溶液的管中。用无菌剪刀将组织切成碎片。
然后将组织碎片在 37 摄氏
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