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Please note that all translations are automatically generated. Click here for the English version.
33 Views
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00:58 min
February 2nd, 2024
DOI :
10.3791/201674-v
Transcript
首先在补充有 10% 胎牛血清的 DMEM 培养基中培养 HEK 293 T 细胞。在转染前一天,将 HEK 293 T 细胞接种到六孔板中,以在第二天达到约 60% 的汇合度。然后使用商业转染试剂盒将实验样品细胞转染到带有质粒的六孔板中,用于 NHEJ 或 HR 测定。
对于流式细胞仪校准对照,留下一孔未转染的细胞作为阴性对照。在六孔板中转染细胞,使用质粒进行 GFP(单色对照)或 mCherry(单色对照)。
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