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एकल सेल ट्रांसक्रिप्टोमिक्स के लिए विभेदित 3 डी मानव आंतों के ऑर्गेनोइड से एकल सेल निलंबन की तैयारी

26 Views

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02:22 min

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June 28th, 2024

DOI :

10.3791/201686-v

June 28th, 2024

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26 Views

Carolyn Bomidi¹, Xi-Lei Zeng¹, Victoria Poplaski¹, Cristian Coarfa², Mary K. Estes¹, Sarah E. Blutt¹

¹Department of Molecular Virology and Microbiology, Baylor College of Medicine, ²Department of Molecular and Cellular Biology, Baylor College of Medicine

Transcript

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Single Cell Suspensions

Differentiated Organoids

Human Intestinal Organoids

Cell Recovery Solution

Enzymatic Cell Digestion

Cell Centrifugation

Trypan Blue Exclusion

Viable Cell Count

Differentiation Media

Absorptive Enterocytes

Secretory Cells

From the series

मानव आंतों के ऑर्गेनोइड के साथ सिंगल-सेल ट्रांसक्रिप्टोमिक्स को एकीकृत करना

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