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Preparation of Single Cell Suspensions from Differentiated 3D Human Intestinal Organoids(단세포 전사체학을 위한 분화된 3D 인간 장 오가노이드에서 단세포 현탁액 준비)

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02:22 min

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June 28th, 2024

DOI :

10.3791/201686-v

June 28th, 2024

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Carolyn Bomidi¹, Xi-Lei Zeng¹, Victoria Poplaski¹, Cristian Coarfa², Mary K. Estes¹, Sarah E. Blutt¹

¹Department of Molecular Virology and Microbiology, Baylor College of Medicine, ²Department of Molecular and Cellular Biology, Baylor College of Medicine

Transcript

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Single Cell Suspensions

Differentiated Organoids

Human Intestinal Organoids

Cell Recovery Solution

Enzymatic Cell Digestion

Cell Centrifugation

Trypan Blue Exclusion

Viable Cell Count

Differentiation Media

Absorptive Enterocytes

Secretory Cells

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Single-Cell Transcriptomics와 Human Intestinal Organoids의 통합

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