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Получение суспензий одиночных клеток из дифференцированных 3D органоидов кишечника человека для транскриптомики одиночных клеток

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29 Views

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02:22 min

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June 28th, 2024

DOI :

10.3791/201686-v

June 28th, 2024

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Carolyn Bomidi¹, Xi-Lei Zeng¹, Victoria Poplaski¹, Cristian Coarfa², Mary K. Estes¹, Sarah E. Blutt¹

¹Department of Molecular Virology and Microbiology, Baylor College of Medicine, ²Department of Molecular and Cellular Biology, Baylor College of Medicine

Transcript

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Single Cell Suspensions

Differentiated Organoids

Human Intestinal Organoids

Cell Recovery Solution

Enzymatic Cell Digestion

Cell Centrifugation

Trypan Blue Exclusion

Viable Cell Count

Differentiation Media

Absorptive Enterocytes

Secretory Cells

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Интеграция транскриптомики одиночных клеток с органоидами кишечника человека

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