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Preparation of single Cell suspensions from Human Intestinal Organoids: Differentiated on Membrane Cell Culture Insert(막 세포 배양 삽입물에서 분화된 인간 장 오가노이드의 단일 세포 현탁액 준비)

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02:36 min

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June 28th, 2024

DOI :

10.3791/201687-v

June 28th, 2024

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Carolyn Bomidi¹, Xi-Lei Zeng¹, Victoria Poplaski¹, Cristian Coarfa², Mary K. Estes¹, Sarah E. Blutt¹

¹Department of Molecular Virology and Microbiology, Baylor College of Medicine, ²Department of Molecular and Cellular Biology, Baylor College of Medicine

Transcript

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Single Cell Suspension

Human Intestinal Organoids

Membrane Cell Culture

Enzymatic Cell Digestion

Differentiation Medium

Cell Dissociation

Micro Centrifuge Tube

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