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01:58 min
June 28th, 2024
DOI :
10.3791/201688-v
Transcript
首先,准备用于人肠道类器官消化的酶细胞消化试剂和 PBS/EDTA。在明场显微镜下,确认分化的三维人类肠道类器官作为单层的生长。丢弃单层培养物中的细胞培养基,并用 100 微升 PBS/EDTA 代替。
去除 PBS/EDTA 后,向每个孔中加入 100 μL 温酶细胞消化试剂。将板置于 37 摄氏度的 5% 二氧化碳培养箱中,每 10 分钟移液 5 次,持续 20 至 30 分钟。每 10 分钟将 1 至 2
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