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Please note that all translations are automatically generated. Click here for the English version.
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03:00 min
April 19th, 2024
DOI :
10.3791/201690-v
Transcript
首先,将小鼠随机分配到对照组或重复轻度创伤性脑损伤组。实验前一周将小鼠适应不同的笼子。仔细检查改进的流体冲击装置是否有泄漏迹象,并确保系统内没有气泡。
麻醉和修剪小鼠头上的皮毛后,用 75% 乙醇对头皮进行消毒。使用立体定位框架中的非末端耳杆固定鼠标,并在下方放置等温加热垫,以将体温保持在 37 摄氏度。使用浸有盐水的棉签仔细清洁手术区域。
沿头皮中线切开 1.5 厘米后,使用平头微钻头和显微手术刀
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