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01:46 min
May 17th, 2024
DOI :
10.3791/201692-v
Transcript
为了将从基于 CRISPR 的模块化组装中获得的 UAS cDNA/ORF 质粒产物转化为大肠杆菌,请在 4 摄氏度下预冷 10 微升尖端。此外,将 1.5 毫升管放入铝冷却块中,在冰上预冷。在冰上解冻感受态大肠杆菌细胞五分钟后,将 10 微升细胞的等分试样加入每个预冷的 1.5 毫升管中。
然后向含有 10 微升感受态细胞的每管中加入 1 微升 UAS cDNA/ORF 质粒产物。轻轻旋转试管混合,然后将其放在冰上 30 分钟。将试管在 42 摄氏度的水浴中孵育一分钟。
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