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Please note that all translations are automatically generated. Click here for the English version.
18 Views
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01:55 min
May 10th, 2024
DOI :
10.3791/201693-v
Transcript
首先,采集浸入角质形成细胞无血清培养基 (KSFM) 中的食管活检样本。用 1 毫升分散酶 I 替换 KSFM,并在室温下孵育活检 10 分钟。之后,使用 1, 000 微升移液管,在不接触活检或细胞碎片颗粒的情况下吸出分散酶。
然后,用DPBS冲洗活检。离心活检后,使用1,000微升移液管吸出上清液。接下来,向活检中加入 500 微升胰蛋白酶-EDTA,并在 37 摄氏度下孵育 10 分钟,同时以 800 rpm 的速度振荡。
然后,进行重复移液,直到获得单细胞悬
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