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00:44 min
May 10th, 2024
DOI :
10.3791/201694-v
Transcript
首先,从食管活检中分离出细胞,并将它们重悬于 4 毫升 KSFM 中,含 10 微摩尔 Y27632。将细胞转移到 T25 细胞培养瓶中。一旦角质形成细胞形成单层,汇合率为 60%至 80%,则传代 P1 细胞。
然后将 P2 原代角质形成细胞接种到 TRANSWELL 插入物上进行气液界面培养。为了在第七天进行气运,用高钙 KSFM 替换下腔室中的培养基,并从上腔室吸出培养基。
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