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Please note that all translations are automatically generated. Click here for the English version.
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04:46 min
May 3rd, 2024
DOI :
10.3791/201706-v
Transcript
首先,获得分化的 THP-1 细胞并从培养板的孔中取出用过的培养基 用 PBS 洗涤细胞后,向细胞中加入含有脂多糖或 LPS 的无血清培养基并孵育板。然后将三磷酸腺苷或ATP储备溶液加入模型组中,并将板在37摄氏度下与5%二氧化碳孵育45分钟。接下来,从培养板的孔中吸出所有上清液。
用 PBS 洗涤细胞两次后,向每个孔中加入 500 微升不含 EDTA 的 0.05% 胰蛋白酶,并孵育 30 秒。为了阻止细胞脱离,加入 1 毫升 RPMI 1640 培养基并将细胞转移到 5 毫
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