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Please note that all translations are automatically generated. Click here for the English version.
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02:11 min
May 24th, 2024
DOI :
10.3791/201758-v
Transcript
首先,在 II A 类生物安全柜内擦拭含有单形革兰氏阴性生物体和 70% 异丙醇的自动血培养瓶的隔膜。然后,将 1 毫升血汤混合物吸入配备 21 号针头的无菌注射器中。将一大滴混合物分配到电镀介质的表面。
划线板后,将培养物在 37 摄氏度下孵育 18 至 24 小时。检查生物安全柜内是否有分离的菌落。接下来,将 3 毫升无菌生理盐水分配到带有分配瓶的 aMIAST 管中。
现在用无菌直接种丝拾取 3 到 5 个形态相似的菌落。将接种物转移到第一管中。使用盐水和密度
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