Research
Education
Solutions
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Türkçe
JA - 日本語
PL - Polski
RU - Русский
Please note that all translations are automatically generated. Click here for the English version.
27 Views
•
02:25 min
December 1st, 2023
DOI :
10.3791/201775-v
Transcript
首先,将从 Notexin 受伤的小鼠中分离的骨骼肌细胞重悬于一毫升冷血清游离 DMEM 中,并使用血细胞计数器或细胞计数器对其进行计数。在通风橱下,加入顺铂原液以达到 25 微摩尔的最终浓度。涡旋试管 10 秒,并在室温下孵育 1 分钟。
用含有 10% FBS 的冰冷 DMEM 淬灭反应,并将其置于冰上。沉淀并重悬细胞后,通过 35 微米细胞过滤器过滤悬浮液。在通风橱下,加入过滤后的多聚甲醛储备液以固定细胞
Sign in or start your free trial to access this content
Explore More Videos
From the series
肌肉再生中生肌祖细胞的单细胞 CyTOF 分析
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2025 MyJoVE Corporation. All rights reserved