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Please note that all translations are automatically generated. Click here for the English version.
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03:26 min
December 1st, 2023
DOI :
10.3791/201776-v
Transcript
首先,取用顺铂、金属偶联抗体和插层铱溶液染色的骨骼肌细胞,并通过离心沉淀细胞。去除上清液后,向涡旋细胞中加入 1 毫升 CSM。通过离心沉淀细胞并在 1 mL CAS 缓冲液中洗涤。
离心后,将上清液吸出约 200 μL。向涡旋样品中加入 1 mL CAS 缓冲液,分装 5 μL 用于细胞计数。离心细胞并将上清液吸出至 50 μL 后,将细胞重悬于 CAS 缓冲液中至终浓度为 100 万个细胞/mL,并添加校准
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