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Please note that all translations are automatically generated. Click here for the English version.
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06:33 min
May 3rd, 2024
DOI :
10.3791/201777-v
Transcript
首先,用70%乙醇喷洒小鼠皮肤。然后,使用无菌解剖剪刀,打开皮肤,露出腿部肌肉。切开腿部旁边的肌肉,以清楚地看到骨头,然后轻轻地去除骨头,而不会损坏它。
立即将骨头放入含有 4% 三聚甲醛的管子中,然后将管子放在冰上。收集所有骨样本后,将试管在 4 摄氏度的冷藏室中置于轨道振荡器上至少 24 小时。固定后,从轨道振荡器中取出骨组织,并用1X PBS洗涤两次,每次3分钟。
然后,使用无菌解剖剪刀去除附着在骨头上的任何剩余肌肉。再次用 1X PBS 清洗骨头三分钟。将骨
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