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Please note that all translations are automatically generated. Click here for the English version.
24 Views
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02:31 min
May 24th, 2024
DOI :
10.3791/201788-v
Transcript
首先,在 DPBS 中用镁和钙制备 1 至 100 稀释的胶原蛋白储备溶液。将 350 微升稀释的储备溶液加入相应的腔室中,并在室温下孵育 5 分钟。使用 350 微升 DPBS 蘸镁和钙冲洗所有腔室两次,以洗掉剩余的胶原蛋白和醋酸。
从一个站点拔下插头并将它们切换到另一侧。再次冲洗腔室后,向其中加入 350 微升内皮细胞生长培养基。从一个站点拔下插头并将它们切换到另一侧。
然后向每个腔室中加入 350 微升内皮细胞生长培养基。取在内皮细胞生长培养基中以 80%至
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