Research
Education
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Turkish
JA - Japanese
Please note that all translations are automatically generated. Click here for the English version.
28 Views
•
02:28 min
June 7th, 2024
DOI :
10.3791/201791-v
Transcript
首先,用画笔将脱氯的果蝇胚胎转移到一条长条的葡萄琼脂上。使用解剖针沿条带边缘排列约 60 个胚胎,它们的后部朝外。现在沿盖玻片的边缘纵向放置一条 18 毫米长的双面胶带。
去除背衬后,轻轻地将盖玻片压在对齐的胚胎上,将带有胚胎的盖玻片转移到装有干燥剂的盒子中。在它们上面添加 halo carbon 油。要显微注射胚胎,首先使用伸出的移液管将 2 微升 DNA 加载到针头中。
将带有卤烃油的对齐胚胎置于倒置显微镜下。接下来,使用显微操作器将针头插入胚胎的后端。使用脚控
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved