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Please note that all translations are automatically generated. Click here for the English version.
18 Views
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02:06 min
May 3rd, 2024
DOI :
10.3791/201793-v
Transcript
首先,将填充的细胞沉淀重悬于等于细胞沉淀体积 7 倍的低渗缓冲液中。将细胞悬液转移到 Potter-Dounce 匀浆器中,在冰上孵育 2 分钟,让细胞膨胀。通过在均质器中执行 8 到 10 次冲程来打破细胞膜,该匀浆器与以 600 RPM 旋转的电机驱动的特氟龙杵相连。
向细胞悬液中加入等体积的高渗缓冲液,以产生等渗环境。将匀浆转移到 10 至 15 毫升的试管中。在 4 摄氏度下以 1, 000G 离心 5 分钟,然后将上清液收集到 1.5 毫升聚丙烯管中。
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