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Please note that all translations are automatically generated. Click here for the English version.
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02:17 min
May 3rd, 2024
DOI :
10.3791/201794-v
Transcript
首先将从哺乳动物细胞培养物或组织获得的线粒体组分重悬于蓝色天然样品缓冲液中,以获得约 10 毫克/毫升的蛋白质浓度。为了溶解线粒体膜,添加洋地黄皂苷以获得每克线粒体蛋白 4 克洋地黄皂苷的比例。轻轻移液混匀,并在冰上孵育 5 分钟。
将悬浮液放入微量离心机中,在 20, 000 G 下在 4 摄氏度下离心 25 分钟,以去除不溶性物质。将上清液收集在新管中,然后加入 5% 考马斯蓝 G-250,相当于初始重悬体积的三分之一,并通过移液混合。将冷阴极 A 缓冲液加入电泳仪的上腔室
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