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Please note that all translations are automatically generated. Click here for the English version.
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01:41 min
December 22nd, 2023
DOI :
10.3791/201802-v
Transcript
首先在无菌条件下用不同的三唑类杀虫剂处理收集的胡萝卜愈伤组织。为此,在单独的玻璃瓶中,将 3 克胡萝卜愈伤组织与 10 毫升在 MS 培养基中制备的每种农药溶液混合。此外,在另一个玻璃瓶中将 3 克胡萝卜愈伤组织与 10 毫升无菌 MS 培养基混合。
将准备好的胡萝卜愈伤组织样品在 130 rpm 和 26 摄氏度下在黑暗中孵育 72 小时。将所有处理一式三份。孵育后,使用 0.5 微米玻璃纤维过滤器,通过过滤
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