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Please note that all translations are automatically generated. Click here for the English version.
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01:35 min
December 22nd, 2023
DOI :
10.3791/201805-v
Transcript
继续执行曝光测试。一旦收获了足够的细胞,轻轻敲击含有细胞的培养瓶壁以使其均匀分布,然后将细胞悬液转移到 15 mL离心管中。用 DMEM 结合细胞术稀释细胞悬液,以达到所需的细胞密度。
轻轻敲击培养瓶壁以确保细胞均匀分布。接下来,向 96 孔板的外孔中加入 100 微升 PBS,以防止培养基因边缘效应而蒸发。将 100 μL 细胞悬液添加到板的左侧孔中,静置 10 分钟。
将细胞在 37 摄氏度和
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