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Please note that all translations are automatically generated. Click here for the English version.
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01:11 min
December 22nd, 2023
DOI :
10.3791/201806-v
Transcript
首先,在暴露于代谢物提取物溶液 24 小时后,从培养箱中取出含有细胞的 96 孔板。处理培养基并使用 PBS 对细胞进行双重洗涤。向每个孔中加入 100 μL DMEM,然后加入 10 μL CCK-8 试剂。
通过轻轻摇动板确保细胞均匀分布。将细胞在 37 摄氏度的 5% 二氧化碳中孵育 4 小时。最后,使用荧光分光光度计在 450 纳米处进行光密度测量。
农药代谢物组和对照组细胞活力差异不显著,
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