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Please note that all translations are automatically generated. Click here for the English version.
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02:08 min
September 6th, 2024
DOI :
10.3791/201869-v
Transcript
首先,将 15 mL 密度梯度培养基放入 15 mL离心管中,并在 20 °C 下以 1, 000 G 离心 1 分钟。倒置含有肝素中采集的人血的试管,并使用经过火灭菌的镊子,取下采血管的盖子。将大约 10 至 15 mL 的血液分配到试管中的密度梯度介质中。
将离心机的减速水平设置为 1,并在室温下将装有血液的试管以 1, 000 G 离心 10 分钟。去除血沉棕黄层上方 1 厘米处的血浆顶层。将离心管中剩余的上清液倒入另一个含有 10 ml PBS 的离心管中。
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