treatment of methionine-induced h-type hypertensive rats with huotan jiedu tongluo decoction (htjdtld)

Antihypertensive virkninger af Huotan Jiedu Tongluo hos H-type hypertensive rotter

Hypertension, a major risk factor for heart attack, stroke, and renal failure, has become a significant public health challenge that affects 1 billion people worldwide1. Homocysteine (Hcy), a thiol group-containing amino acid, is a vital metabolic intermediary of methionine metabolism. Hypertension with elevated plasma Hcy levels is defined as H-type hypertension, which could be a significant risk factor for the occurrence and recurrence of cardiocerebrovascular diseases such as stroke2,3. Recent studies have reported that the co-residency of H-type hypertension could aggravate the side effects of cardiovascular and cerebrovascular diseases4. Notably, 75% of patients in China with H-type hypertension have primary hypertension, which seriously affects the quality of life5. At present, the treatment of H-type hypertension mainly includes Western medicine. However, it may cause certain adverse effects and poor compliance and can no longer meet the needs for the comprehensive management of H-type hypertension.
Traditional Chinese medicine (TCM) is a unique resource with a history of more than 2,000 years in China. Due to the unmet need for hypertension control in Western medicine, clinicians have begun to consider the potential role of TCM in the prevention and treatment of H-type hypertension6. Huotan Jiedu Tongluo Decoction (HTJDTLD) is a traditional Chinese medicine formula formulated by Professor Yue Deng, drawing from his extensive clinical expertise7. Over the course of more than 20 years of clinical application, HTJDTLD has demonstrated remarkable effectiveness in the treatment of cardiovascular and cerebrovascular diseases1. However, whether HTJDTLD has therapeutic effects in H-type hypertension has not been reported. Therefore, we aimed to explore the antihypertensive effects and specific mechanisms of HTJDTLD in rats with H-type hypertension and identify potential therapeutic drugs for the treatment of H-type hypertension.

Forfatter Spotlight: Udforskning af Huotan Jiedu Tongluo afkog som et antihypertensivt lægemiddel

De antihypertensive virkninger og mekanismer af Huotan Jiedu Tongluo afkog hos rotter med H-type hypertension

H-type hypertension has become a significant public health challenge of human. It mainly relies on Western medicine treatment, which can have sad effects. Our research explores the anti-hypertensive effects and mechanisms of Huotan Jiedu Tongluo decoction, a traditional Chinese medicine formula, in rats with H-type hypertension.Our study indicated that Huotan Jiedu Tongluo decoction had anti-hypertensive effects in rats with H-type hypertension. The underlying mechanism may involve inhibiting ER stress activation-induced apoptosis which provide an effective treatment option for patients. We will explode the potential biomarkers for early diagnosis and prognosis and develop novel therapeutic drugs for H-type hypertension in the future.

Behandling af methionin-inducerede H-type hypertensive rotter med Huotan Jiedu Tongluo afkog (HTJDTLD)

Til at begynde med skal du adskille 50 hypertensive rotter i fem grupper, inklusive kontrol. Giv rotterne i gruppe 1, methionin eller met-gruppen, en diæt på 3%methionin i 28 dage for at fremkalde H-type hypertension. Til gruppe 2 til 4 rotter administreres en kombination af Huotan Jiedu Tongluo Decoction og enalapril maleat ud over methionin-diæten i 28 dage.Opret derefter en fastholdelsesanordning ved at placere et cylindrisk fastholdelsesnet i et termisk rør og sætte det termiske rør i et lærredsdæksel. Efter 28 dages behandling skal du placere en rotte i masken. Placer derefter signalkablet til et ikke-invasivt blodtryksmåler under dækslet.Placer rottens hale i mellemrummet i dækslet og fastgør den på den stabiliserende formpude. Overfør rotten til et stille målested 20 til 30 minutter før blodtryksmåling. Tilslut nu tryksensorens luftslangeforbindelse, signalforbindelse og holderør.Placer tryksensoren på spidsen af rottens hale. En pulsbølge skal vises, efter at rottehalen er indsat i sensoren. Tryk på Start/Stop for at starte eller stoppe målingen.Når blodtrykstesten er afsluttet, vises resultatmenuen automatisk. Kontroller gennemsnitsværdien af målingen, standardafvigelsen, standarden og variationskoefficienten i resultatmenuen. Efter aflivning af rotten dissekeres den med en kirurgisk saks og tandpincet fra mellemkødet til halsen.Vend bryst- og maveindholdet. Naviger derefter til abdominal aorta-bifurkation mellem de to nyrer. Saml aorta op til en sektion af aortabuen.Det systoliske og diastoliske blodtryk var signifikant højere i Met-gruppen i forhold til kontrolgruppen fra en til fire uger. Blodtrykket faldt efter administration af Huotan Jiedu Tongluo afkog. Den kombinerede virkning af afkoget og/eller enalaprilmaleatet havde en stærkere antihypertensiv virkning end afkoget alene.

Research

JoVE Journal

Biochemistry

Treatment of Methionine-Induced H-Type Hypertensive Rats with Huotan Jiedu Tongluo Decoction (HTJDTLD)

Antihypertensive Effects of Huotan Jiedu Tongluo in H-Type Hypertensive Rats

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension

Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health ...

Plasma Homocysteine (Hcy) ELISA Measurement of Blood from H-Type Hypertensive Rats Treated with Huotan Jiedu Tongluo Decoction

Plasmahomocystein (Hcy) ELISA-måling af blod fra H-type hypertensive rotter behandlet med Huotan Jiedu Tongluo afkog