treatment of methionine-induced h-type hypertensive rats with huotan jiedu tongluo decoction (htjdtld)

Antihypertensive effekter av Huotan Jiedu Tongluo hos H-type hypertensive rotter

Hypertension, a major risk factor for heart attack, stroke, and renal failure, has become a significant public health challenge that affects 1 billion people worldwide1. Homocysteine (Hcy), a thiol group-containing amino acid, is a vital metabolic intermediary of methionine metabolism. Hypertension with elevated plasma Hcy levels is defined as H-type hypertension, which could be a significant risk factor for the occurrence and recurrence of cardiocerebrovascular diseases such as stroke2,3. Recent studies have reported that the co-residency of H-type hypertension could aggravate the side effects of cardiovascular and cerebrovascular diseases4. Notably, 75% of patients in China with H-type hypertension have primary hypertension, which seriously affects the quality of life5. At present, the treatment of H-type hypertension mainly includes Western medicine. However, it may cause certain adverse effects and poor compliance and can no longer meet the needs for the comprehensive management of H-type hypertension.
Traditional Chinese medicine (TCM) is a unique resource with a history of more than 2,000 years in China. Due to the unmet need for hypertension control in Western medicine, clinicians have begun to consider the potential role of TCM in the prevention and treatment of H-type hypertension6. Huotan Jiedu Tongluo Decoction (HTJDTLD) is a traditional Chinese medicine formula formulated by Professor Yue Deng, drawing from his extensive clinical expertise7. Over the course of more than 20 years of clinical application, HTJDTLD has demonstrated remarkable effectiveness in the treatment of cardiovascular and cerebrovascular diseases1. However, whether HTJDTLD has therapeutic effects in H-type hypertension has not been reported. Therefore, we aimed to explore the antihypertensive effects and specific mechanisms of HTJDTLD in rats with H-type hypertension and identify potential therapeutic drugs for the treatment of H-type hypertension.

Forfatter Spotlight: Utforske Huotan Jiedu Tongluo avkok som et antihypertensivt legemiddel

De antihypertensive effektene og mekanismene til Huotan Jiedu Tongluo-avkok hos rotter med H-type hypertensjon

H-type hypertension has become a significant public health challenge of human. It mainly relies on Western medicine treatment, which can have sad effects. Our research explores the anti-hypertensive effects and mechanisms of Huotan Jiedu Tongluo decoction, a traditional Chinese medicine formula, in rats with H-type hypertension.Our study indicated that Huotan Jiedu Tongluo decoction had anti-hypertensive effects in rats with H-type hypertension. The underlying mechanism may involve inhibiting ER stress activation-induced apoptosis which provide an effective treatment option for patients. We will explode the potential biomarkers for early diagnosis and prognosis and develop novel therapeutic drugs for H-type hypertension in the future.

Behandling av metionininduserte H-type hypertensive rotter med Huotan Jiedu Tongluo avkok (HTJDTLD)

Til å begynne med, segreger 50 hypertensive rotter i fem grupper, inkludert kontroll. Gi rottene i gruppe 1, metionin eller met-gruppe, en diett på 3 % metionin i 28 dager for å indusere hypertensjon av H-typen. For gruppe 2 til 4 rotter, administrer en kombinasjon av Huotan Jiedu Tongluo Decoction og enalaprilmaleat i tillegg til metionindietten i 28 dager.Deretter lager du en fastholdelsesanordning ved å plassere et sylindrisk tilbakeholdelsesnett i et termisk rør og sette det termiske røret i et lerretsdeksel. Etter 28 dagers behandling, plasser en rotte i nettet. Plasser deretter signalkabelen til et ikke-invasivt blodtrykksmåler under dekselet.Plasser rottens hale i gapet i dekselet og fest den på den stabiliserende formputen. Overfør rotta til et rolig målested 20 til 30 minutter før blodtrykksmåling. Koble nå til trykksensorens luftslangetilkobling, signaltilkobling og holderør.Plasser trykksensoren på tuppen av rottens hale. En pulsbølge skal vises etter at rottehalen er satt inn i sensoren. Trykk på Start/Stopp for å starte eller stoppe målingen.Når blodtrykkstesten er fullført, vil resultatmenyen dukke opp automatisk. Sjekk gjennomsnittsverdien av målingen, standardavvik, standard og variasjonskoeffisient i resultatmenyen. Etter å ha avlivet rotten, dissekerer du den med en kirurgisk saks og tanntang fra perineum til nakken.Snu bryst- og mageinnholdet. Naviger deretter til abdominal aorta-bifurkasjonen mellom de to nyrene. Samle aorta opp til en del av aortabuen.Det systoliske og diastoliske blodtrykket var signifikant høyere i Met-gruppen i forhold til kontrollgruppen fra en til fire uker. Blodtrykket sank etter administrering av Huotan Jiedu Tongluo Decoction. Den kombinerte effekten av avkoket og/eller enalaprilmaleatet hadde en sterkere antihypertensiv effekt enn avkoket alene.

Research

JoVE Journal

Biochemistry

Treatment of Methionine-Induced H-Type Hypertensive Rats with Huotan Jiedu Tongluo Decoction (HTJDTLD)

Antihypertensive Effects of Huotan Jiedu Tongluo in H-Type Hypertensive Rats

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension

Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health ...

Plasma Homocysteine (Hcy) ELISA Measurement of Blood from H-Type Hypertensive Rats Treated with Huotan Jiedu Tongluo Decoction

Plasmahomocystein (Hcy) ELISA-måling av blod fra H-type hypertensive rotter behandlet med Huotan Jiedu Tongluo avkok