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Please note that all translations are automatically generated. Click here for the English version.
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02:26 min
May 17th, 2024
DOI :
10.3791/201881-v
Transcript
首先,将凝固的含血管以 626 比 1、409 G 的离心力在 2 至 8 摄氏度下离心 20 分钟。然后小心地将上清液收集在新管中。将上清液储存在零下 80 摄氏度。
接下来,设置空白标准品孔和样品孔。将 50 μL 稀释的标准品移液到 ELISA 板中。然后将 40 微升稀释的样品转移到样品孔中。
向样品孔中加入 10 μL 血清。现在,用密封膜密封板。将密封板放入 37 摄氏度的培养箱中 30 分钟。
用蒸馏水稀释 30 倍浓洗液,得单一强度的洗液
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