plasma homocysteine (hcy) elisa measurement of blood from h-type hypertensive rats treated with huotan jiedu tongluo decoction

Antihypertensive virkninger af Huotan Jiedu Tongluo hos H-type hypertensive rotter

Hypertension, a major risk factor for heart attack, stroke, and renal failure, has become a significant public health challenge that affects 1 billion people worldwide1. Homocysteine (Hcy), a thiol group-containing amino acid, is a vital metabolic intermediary of methionine metabolism. Hypertension with elevated plasma Hcy levels is defined as H-type hypertension, which could be a significant risk factor for the occurrence and recurrence of cardiocerebrovascular diseases such as stroke2,3. Recent studies have reported that the co-residency of H-type hypertension could aggravate the side effects of cardiovascular and cerebrovascular diseases4. Notably, 75% of patients in China with H-type hypertension have primary hypertension, which seriously affects the quality of life5. At present, the treatment of H-type hypertension mainly includes Western medicine. However, it may cause certain adverse effects and poor compliance and can no longer meet the needs for the comprehensive management of H-type hypertension.
Traditional Chinese medicine (TCM) is a unique resource with a history of more than 2,000 years in China. Due to the unmet need for hypertension control in Western medicine, clinicians have begun to consider the potential role of TCM in the prevention and treatment of H-type hypertension6. Huotan Jiedu Tongluo Decoction (HTJDTLD) is a traditional Chinese medicine formula formulated by Professor Yue Deng, drawing from his extensive clinical expertise7. Over the course of more than 20 years of clinical application, HTJDTLD has demonstrated remarkable effectiveness in the treatment of cardiovascular and cerebrovascular diseases1. However, whether HTJDTLD has therapeutic effects in H-type hypertension has not been reported. Therefore, we aimed to explore the antihypertensive effects and specific mechanisms of HTJDTLD in rats with H-type hypertension and identify potential therapeutic drugs for the treatment of H-type hypertension.

Forfatter Spotlight: Udforskning af Huotan Jiedu Tongluo afkog som et antihypertensivt lægemiddel

De antihypertensive virkninger og mekanismer af Huotan Jiedu Tongluo afkog hos rotter med H-type hypertension

H-type hypertension has become a significant public health challenge of human. It mainly relies on Western medicine treatment, which can have sad effects. Our research explores the anti-hypertensive effects and mechanisms of Huotan Jiedu Tongluo decoction, a traditional Chinese medicine formula, in rats with H-type hypertension.Our study indicated that Huotan Jiedu Tongluo decoction had anti-hypertensive effects in rats with H-type hypertension. The underlying mechanism may involve inhibiting ER stress activation-induced apoptosis which provide an effective treatment option for patients. We will explode the potential biomarkers for early diagnosis and prognosis and develop novel therapeutic drugs for H-type hypertension in the future.

Plasmahomocystein (Hcy) ELISA-måling af blod fra H-type hypertensive rotter behandlet med Huotan Jiedu Tongluo afkog

For at begynde med centrifugeres de koagulerede blodholdige rør ved 626 til 1.409 G i 20 minutter ved to til otte grader Celsius. Saml derefter forsigtigt supernatanten i et nyt rør. Opbevar supernatanten ved minus 80 grader Celsius.Opsæt derefter blank standard og prøvebrønde. Pipetter 50 mikroliter af de fortyndede standarder ind i ELISA-pladen. Overfør derefter 40 mikroliter af de fortyndede prøver til prøvebrøndene.Der tilsættes 10 mikroliter serum i prøvebrøndene. Forsegl nu pladen med en tætningsfilm. Placer den forseglede plade i en inkubator ved 37 grader Celsius i 30 minutter.Den 30 gange koncentrerede vaskeopløsning fortyndes med destilleret vand for at opnå en vaskeopløsning med en enkelt styrke. Fjern derefter tætningsfilmen fra pladen og kassér væsken. Fyld nu hver brønd med 200 mikroliter vaskeopløsning fem gange.Når du har kasseret supernatanten for sidste gang, skal du banke pladen tør. Pipetter 50 mikroliter af enzymreagenset i alle andre brønde end de blanke. Forsegl derefter pladen med en forseglingsfilm, inden den placeres i en inkubator ved 37 grader Celsius i 30 minutter.Efter vask af pladen fem gange mere tilsættes 50 mikroliter af hver af farvefremkalderne, A og B.Ryst pladen forsigtigt for at blande godt. Inkuber derefter pladen ved 37 grader Celsius ved svagt lys i 10 minutter. Pipetter nu 50 mikroliter af stopopløsningen i hver brønd for at afslutte reaktionen.Mål absorbansen af hver brønd sekventielt ved 450 nanometer. Plasmakoncentrationen af homocystein i methioningruppen var dobbelt så stor som i kontrolgruppen. Plasmakoncentrationen af homocystein var relativt lavere i behandlingsgrupperne.

Research

JoVE Journal

Biochemistry

Plasma Homocysteine (Hcy) ELISA Measurement of Blood from H-Type Hypertensive Rats Treated with Huotan Jiedu Tongluo Decoction

Antihypertensive Effects of Huotan Jiedu Tongluo in H-Type Hypertensive Rats

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension

Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health ...

Treatment of Methionine-Induced H-Type Hypertensive Rats with Huotan Jiedu Tongluo Decoction (HTJDTLD)

Behandling af methionin-inducerede H-type hypertensive rotter med Huotan Jiedu Tongluo afkog (HTJDTLD)