plasma homocysteine (hcy) elisa measurement of blood from h-type hypertensive rats treated with huotan jiedu tongluo decoction

Effetti antipertensivi di Huotan Jiedu Tongluo nei ratti ipertesi di tipo H

Hypertension, a major risk factor for heart attack, stroke, and renal failure, has become a significant public health challenge that affects 1 billion people worldwide1. Homocysteine (Hcy), a thiol group-containing amino acid, is a vital metabolic intermediary of methionine metabolism. Hypertension with elevated plasma Hcy levels is defined as H-type hypertension, which could be a significant risk factor for the occurrence and recurrence of cardiocerebrovascular diseases such as stroke2,3. Recent studies have reported that the co-residency of H-type hypertension could aggravate the side effects of cardiovascular and cerebrovascular diseases4. Notably, 75% of patients in China with H-type hypertension have primary hypertension, which seriously affects the quality of life5. At present, the treatment of H-type hypertension mainly includes Western medicine. However, it may cause certain adverse effects and poor compliance and can no longer meet the needs for the comprehensive management of H-type hypertension.
Traditional Chinese medicine (TCM) is a unique resource with a history of more than 2,000 years in China. Due to the unmet need for hypertension control in Western medicine, clinicians have begun to consider the potential role of TCM in the prevention and treatment of H-type hypertension6. Huotan Jiedu Tongluo Decoction (HTJDTLD) is a traditional Chinese medicine formula formulated by Professor Yue Deng, drawing from his extensive clinical expertise7. Over the course of more than 20 years of clinical application, HTJDTLD has demonstrated remarkable effectiveness in the treatment of cardiovascular and cerebrovascular diseases1. However, whether HTJDTLD has therapeutic effects in H-type hypertension has not been reported. Therefore, we aimed to explore the antihypertensive effects and specific mechanisms of HTJDTLD in rats with H-type hypertension and identify potential therapeutic drugs for the treatment of H-type hypertension.

Autore in primo piano: Esplorare il decotto di Huotan Jiedu Tongluo come farmaco antipertensivo

Gli effetti antipertensivi e i meccanismi del decotto di Huotan Jiedu Tongluo nei ratti con ipertensione di tipo H

H-type hypertension has become a significant public health challenge of human. It mainly relies on Western medicine treatment, which can have sad effects. Our research explores the anti-hypertensive effects and mechanisms of Huotan Jiedu Tongluo decoction, a traditional Chinese medicine formula, in rats with H-type hypertension.Our study indicated that Huotan Jiedu Tongluo decoction had anti-hypertensive effects in rats with H-type hypertension. The underlying mechanism may involve inhibiting ER stress activation-induced apoptosis which provide an effective treatment option for patients. We will explode the potential biomarkers for early diagnosis and prognosis and develop novel therapeutic drugs for H-type hypertension in the future.

Misurazione ELISA dell'omocisteina plasmatica (Hcy) del sangue di ratti ipertesi di tipo H trattati con decotto di Huotan Jiedu Tongluo

Per iniziare, centrifugare le provette contenenti sangue coagulato a 626-1.409 G per 20 minuti a una temperatura compresa tra due e otto gradi Celsius. Quindi raccogliere con cura il surnatante in un nuovo tubo. Conservare il surnatante a meno 80 gradi Celsius.Successivamente, impostare i pozzetti standard e campionare in bianco. Pipettare 50 microlitri degli standard diluiti nella piastra ELISA. Quindi trasferire 40 microlitri dei campioni diluiti nei pozzetti del campione.Aggiungere 10 microlitri di siero nei pozzetti del campione. Ora, sigillare la piastra con una pellicola sigillante. Mettere il piatto sigillato in un'incubatrice a 37 gradi Celsius per 30 minuti.Diluire la soluzione di lavaggio concentrata 30 volte con acqua distillata per ottenere una soluzione di lavaggio a forza singola. Quindi, rimuovere la pellicola sigillante dalla piastra e scartare il liquido. Ora, riempi ogni pozzetto con 200 microlitri di soluzione di lavaggio cinque volte.Dopo aver scartato il surnatante per l'ultima volta, picchiettare la piastra per asciugarla. Pipettare 50 microlitri di reagente enzimatico in tutti i pozzetti diversi da quelli bianchi. Quindi sigillare la piastra con una pellicola sigillante prima di metterla in un'incubatrice a 37 gradi Celsius per 30 minuti.Dopo aver lavato la piastra altre cinque volte, aggiungere 50 microlitri di ciascuno degli sviluppatori di colore, A e B.Agitare delicatamente la piastra per mescolare bene. Quindi incubare la piastra a 37 gradi Celsius in condizioni di scarsa illuminazione per 10 minuti. Ora, pipettare 50 microlitri della soluzione di arresto in ciascun pozzetto per terminare la reazione.Misurare l'assorbanza di ciascun pozzetto in sequenza a 450 nanometri. La concentrazione plasmatica di omocisteina nel gruppo metionina era due volte maggiore rispetto al gruppo di controllo. Le concentrazioni plasmatiche di omocisteina erano relativamente più basse nei gruppi di trattamento.

Research

JoVE Journal

Biochemistry

Plasma Homocysteine (Hcy) ELISA Measurement of Blood from H-Type Hypertensive Rats Treated with Huotan Jiedu Tongluo Decoction

Antihypertensive Effects of Huotan Jiedu Tongluo in H-Type Hypertensive Rats

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension

Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health ...

Ricerca

Biochimica

Treatment of Methionine-Induced H-Type Hypertensive Rats with Huotan Jiedu Tongluo Decoction (HTJDTLD)

Trattamento di ratti ipertesi di tipo H indotti da metionina con decotto di Huotan Jiedu Tongluo (HTJDTLD)