plasma homocysteine (hcy) elisa measurement of blood from h-type hypertensive rats treated with huotan jiedu tongluo decoction

Antihypertensive effekter av Huotan Jiedu Tongluo hos H-type hypertensive rotter

Hypertension, a major risk factor for heart attack, stroke, and renal failure, has become a significant public health challenge that affects 1 billion people worldwide1. Homocysteine (Hcy), a thiol group-containing amino acid, is a vital metabolic intermediary of methionine metabolism. Hypertension with elevated plasma Hcy levels is defined as H-type hypertension, which could be a significant risk factor for the occurrence and recurrence of cardiocerebrovascular diseases such as stroke2,3. Recent studies have reported that the co-residency of H-type hypertension could aggravate the side effects of cardiovascular and cerebrovascular diseases4. Notably, 75% of patients in China with H-type hypertension have primary hypertension, which seriously affects the quality of life5. At present, the treatment of H-type hypertension mainly includes Western medicine. However, it may cause certain adverse effects and poor compliance and can no longer meet the needs for the comprehensive management of H-type hypertension.
Traditional Chinese medicine (TCM) is a unique resource with a history of more than 2,000 years in China. Due to the unmet need for hypertension control in Western medicine, clinicians have begun to consider the potential role of TCM in the prevention and treatment of H-type hypertension6. Huotan Jiedu Tongluo Decoction (HTJDTLD) is a traditional Chinese medicine formula formulated by Professor Yue Deng, drawing from his extensive clinical expertise7. Over the course of more than 20 years of clinical application, HTJDTLD has demonstrated remarkable effectiveness in the treatment of cardiovascular and cerebrovascular diseases1. However, whether HTJDTLD has therapeutic effects in H-type hypertension has not been reported. Therefore, we aimed to explore the antihypertensive effects and specific mechanisms of HTJDTLD in rats with H-type hypertension and identify potential therapeutic drugs for the treatment of H-type hypertension.

Forfatter Spotlight: Utforske Huotan Jiedu Tongluo avkok som et antihypertensivt legemiddel

De antihypertensive effektene og mekanismene til Huotan Jiedu Tongluo-avkok hos rotter med H-type hypertensjon

H-type hypertension has become a significant public health challenge of human. It mainly relies on Western medicine treatment, which can have sad effects. Our research explores the anti-hypertensive effects and mechanisms of Huotan Jiedu Tongluo decoction, a traditional Chinese medicine formula, in rats with H-type hypertension.Our study indicated that Huotan Jiedu Tongluo decoction had anti-hypertensive effects in rats with H-type hypertension. The underlying mechanism may involve inhibiting ER stress activation-induced apoptosis which provide an effective treatment option for patients. We will explode the potential biomarkers for early diagnosis and prognosis and develop novel therapeutic drugs for H-type hypertension in the future.

Plasmahomocystein (Hcy) ELISA-måling av blod fra H-type hypertensive rotter behandlet med Huotan Jiedu Tongluo avkok

For å begynne, sentrifuger de koagulerte blodholdige rørene ved 626 til 1, 409 G i 20 minutter ved to til åtte grader Celsius. Samle deretter supernatanten forsiktig i et nytt rør. Oppbevar supernatanten ved minus 80 grader Celsius.Deretter setter du opp blank standard og prøvebrønner. Pipetter 50 mikroliter av de fortynnede standardene inn i ELISA-platen. Overfør deretter 40 mikroliter av de fortynnede prøvene til prøvebrønnene.Tilsett 10 mikroliter serum i prøvebrønnene. Forsegl nå platen med en tetningsfilm. Plasser den forseglede platen i en inkubator ved 37 grader Celsius i 30 minutter.Fortynn den 30 ganger konsentrerte vaskeløsningen med destillert vann for å oppnå en vaskeløsning med én styrke. Fjern deretter tetningsfilmen fra platen og kast væsken. Fyll nå hver brønn med 200 mikroliter vaskeløsning fem ganger.Etter å ha kastet supernatanten for siste gang, bank platen tørr. Pipetter 50 mikroliter av enzymreagenset i alle andre brønner enn de blanke. Forsegl deretter platen med en tetningsfilm før du legger den i en inkubator ved 37 grader Celsius i 30 minutter.Etter å ha vasket platen fem ganger til, tilsett 50 mikroliter av hver av fargefremkallerne, A og B.Rist platen forsiktig for å blande godt. Inkuber deretter platen ved 37 grader Celsius ved lite lys i 10 minutter. Pipetter nå 50 mikroliter av stoppløsningen i hver brønn for å avslutte reaksjonen.Mål absorbansen til hver brønn sekvensielt ved 450 nanometer. Plasmakonsentrasjonen av homocystein i metioningruppen var dobbelt så stor som i kontrollgruppen. Plasmakonsentrasjonen av homocystein var relativt lavere i behandlingsgruppene.

Research

JoVE Journal

Biochemistry

Plasma Homocysteine (Hcy) ELISA Measurement of Blood from H-Type Hypertensive Rats Treated with Huotan Jiedu Tongluo Decoction

Antihypertensive Effects of Huotan Jiedu Tongluo in H-Type Hypertensive Rats

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension

Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health ...

Treatment of Methionine-Induced H-Type Hypertensive Rats with Huotan Jiedu Tongluo Decoction (HTJDTLD)

Behandling av metionininduserte H-type hypertensive rotter med Huotan Jiedu Tongluo avkok (HTJDTLD)