plasma homocysteine (hcy) elisa measurement of blood from h-type hypertensive rats treated with huotan jiedu tongluo decoction

Efectos antihipertensivos de Huotan Jiedu Tongluo en ratas hipertensas tipo H

Hypertension, a major risk factor for heart attack, stroke, and renal failure, has become a significant public health challenge that affects 1 billion people worldwide1. Homocysteine (Hcy), a thiol group-containing amino acid, is a vital metabolic intermediary of methionine metabolism. Hypertension with elevated plasma Hcy levels is defined as H-type hypertension, which could be a significant risk factor for the occurrence and recurrence of cardiocerebrovascular diseases such as stroke2,3. Recent studies have reported that the co-residency of H-type hypertension could aggravate the side effects of cardiovascular and cerebrovascular diseases4. Notably, 75% of patients in China with H-type hypertension have primary hypertension, which seriously affects the quality of life5. At present, the treatment of H-type hypertension mainly includes Western medicine. However, it may cause certain adverse effects and poor compliance and can no longer meet the needs for the comprehensive management of H-type hypertension.
Traditional Chinese medicine (TCM) is a unique resource with a history of more than 2,000 years in China. Due to the unmet need for hypertension control in Western medicine, clinicians have begun to consider the potential role of TCM in the prevention and treatment of H-type hypertension6. Huotan Jiedu Tongluo Decoction (HTJDTLD) is a traditional Chinese medicine formula formulated by Professor Yue Deng, drawing from his extensive clinical expertise7. Over the course of more than 20 years of clinical application, HTJDTLD has demonstrated remarkable effectiveness in the treatment of cardiovascular and cerebrovascular diseases1. However, whether HTJDTLD has therapeutic effects in H-type hypertension has not been reported. Therefore, we aimed to explore the antihypertensive effects and specific mechanisms of HTJDTLD in rats with H-type hypertension and identify potential therapeutic drugs for the treatment of H-type hypertension.

Autor destacado: Explorando la decocción de Huotan Jiedu Tongluo como medicamento antihipertensivo

Los efectos antihipertensivos y los mecanismos de la decocción de Huotan Jiedu Tongluo en ratas con hipertensión tipo H

H-type hypertension has become a significant public health challenge of human. It mainly relies on Western medicine treatment, which can have sad effects. Our research explores the anti-hypertensive effects and mechanisms of Huotan Jiedu Tongluo decoction, a traditional Chinese medicine formula, in rats with H-type hypertension.Our study indicated that Huotan Jiedu Tongluo decoction had anti-hypertensive effects in rats with H-type hypertension. The underlying mechanism may involve inhibiting ER stress activation-induced apoptosis which provide an effective treatment option for patients. We will explode the potential biomarkers for early diagnosis and prognosis and develop novel therapeutic drugs for H-type hypertension in the future.

Medición ELISA de homocisteína (Hcy) en plasma de sangre de ratas hipertensas tipo H tratadas con la decocción de Huotan Jiedu Tongluo

Para comenzar, centrifugar los tubos que contienen sangre coagulada a 626 a 1.409 G durante 20 minutos a dos a ocho grados Celsius. A continuación, recoja con cuidado el sobrenadante en un tubo nuevo. Almacene el sobrenadante a menos 80 grados centígrados.A continuación, configure pocillos estándar y de muestra en blanco. Pipetear 50 microlitros de los patrones diluidos en la placa ELISA. A continuación, transfiera 40 microlitros de las muestras diluidas a los pocillos de muestreo.Agregue 10 microlitros de suero en los pocillos de muestra. Ahora, selle la placa con una película de sellado. Coloque la placa sellada en una incubadora a 37 grados centígrados durante 30 minutos.Diluya la solución de lavado 30 veces concentrada con agua destilada para obtener una solución de lavado de concentración única. A continuación, retire la película de sellado de la placa y deseche el líquido. Ahora, llene cada pocillo con 200 microlitros de solución de lavado cinco veces.Después de desechar el sobrenadante por última vez, seque la placa con golpecitos. Pipetear 50 microlitros del reactivo enzimático en todos los pocillos que no sean los blancos. Luego selle la placa con una película selladora antes de colocarla en una incubadora a 37 grados centígrados durante 30 minutos.Después de lavar la placa cinco veces más, agregue 50 microlitros de cada uno de los reveladores de color, A y B. Agite la placa suavemente para mezclar bien. A continuación, incubar la placa a 37 grados centígrados con poca luz durante 10 minutos. Ahora, pipetee 50 microlitros de la solución de parada en cada pocillo para terminar la reacción.Mida la absorbancia de cada pocillo secuencialmente a 450 nanómetros. La concentración plasmática de homocisteína en el grupo de metionina fue dos veces mayor que en el grupo control. Las concentraciones plasmáticas de homocisteína fueron relativamente más bajas en los grupos de tratamiento.

Research

JoVE Journal

Biochemistry

Plasma Homocysteine (Hcy) ELISA Measurement of Blood from H-Type Hypertensive Rats Treated with Huotan Jiedu Tongluo Decoction

Antihypertensive Effects of Huotan Jiedu Tongluo in H-Type Hypertensive Rats

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension

Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health ...

Investigación

Bioquímica

Treatment of Methionine-Induced H-Type Hypertensive Rats with Huotan Jiedu Tongluo Decoction (HTJDTLD)

Tratamiento de ratas hipertensas tipo H inducidas por metionina con decocción de Huotan Jiedu Tongluo (HTJDTLD)