plasma homocysteine (hcy) elisa measurement of blood from h-type hypertensive rats treated with huotan jiedu tongluo decoction

Antihypertensiva effekter av Huotan Jiedu Tongluo hos hypertensiva råttor av H-typ

Hypertension, a major risk factor for heart attack, stroke, and renal failure, has become a significant public health challenge that affects 1 billion people worldwide1. Homocysteine (Hcy), a thiol group-containing amino acid, is a vital metabolic intermediary of methionine metabolism. Hypertension with elevated plasma Hcy levels is defined as H-type hypertension, which could be a significant risk factor for the occurrence and recurrence of cardiocerebrovascular diseases such as stroke2,3. Recent studies have reported that the co-residency of H-type hypertension could aggravate the side effects of cardiovascular and cerebrovascular diseases4. Notably, 75% of patients in China with H-type hypertension have primary hypertension, which seriously affects the quality of life5. At present, the treatment of H-type hypertension mainly includes Western medicine. However, it may cause certain adverse effects and poor compliance and can no longer meet the needs for the comprehensive management of H-type hypertension.
Traditional Chinese medicine (TCM) is a unique resource with a history of more than 2,000 years in China. Due to the unmet need for hypertension control in Western medicine, clinicians have begun to consider the potential role of TCM in the prevention and treatment of H-type hypertension6. Huotan Jiedu Tongluo Decoction (HTJDTLD) is a traditional Chinese medicine formula formulated by Professor Yue Deng, drawing from his extensive clinical expertise7. Over the course of more than 20 years of clinical application, HTJDTLD has demonstrated remarkable effectiveness in the treatment of cardiovascular and cerebrovascular diseases1. However, whether HTJDTLD has therapeutic effects in H-type hypertension has not been reported. Therefore, we aimed to explore the antihypertensive effects and specific mechanisms of HTJDTLD in rats with H-type hypertension and identify potential therapeutic drugs for the treatment of H-type hypertension.

Författare i fokus: Utforska Huotan Jiedu Tongluo avkok som ett blodtryckssänkande läkemedel

De blodtryckssänkande effekterna och mekanismerna för Huotan Jiedu Tongluo avkok hos råttor med H-typ hypertoni

H-type hypertension has become a significant public health challenge of human. It mainly relies on Western medicine treatment, which can have sad effects. Our research explores the anti-hypertensive effects and mechanisms of Huotan Jiedu Tongluo decoction, a traditional Chinese medicine formula, in rats with H-type hypertension.Our study indicated that Huotan Jiedu Tongluo decoction had anti-hypertensive effects in rats with H-type hypertension. The underlying mechanism may involve inhibiting ER stress activation-induced apoptosis which provide an effective treatment option for patients. We will explode the potential biomarkers for early diagnosis and prognosis and develop novel therapeutic drugs for H-type hypertension in the future.

Plasmahomocystein (Hcy) ELISA-mätning av blod från H-typ hypertensiva råttor behandlade med Huotan Jiedu Tongluo avkok

Till att börja med, centrifugera de koagulerade blodinnehållande rören vid 626 till 1, 409 G i 20 minuter vid två till åtta grader Celsius. Samla sedan försiktigt upp supernatanten i ett nytt rör. Förvara supernatanten vid minus 80 grader Celsius.Ställ sedan in tomma standard- och provbrunnar. Pipettera 50 mikroliter av de utspädda standarderna i ELISA-plattan. Överför sedan 40 mikroliter av de utspädda proverna till provbrunnarna.Tillsätt 10 mikroliter serum i provbrunnarna. Försegla nu plattan med en tätningsfilm. Placera den förseglade plattan i en inkubator vid 37 grader Celsius i 30 minuter.Späd den 30 gånger koncentrerade tvättlösningen med destillerat vatten för att få en tvättlösning med en enda styrka. Ta sedan bort tätningsfilmen från plattan och kassera vätskan. Fyll nu varje brunn med 200 mikroliter tvättlösning fem gånger.Efter att ha kasserat supernatanten för sista gången, knacka plattan torr. Pipettera 50 mikroliter av enzymreagenset i alla brunnar utom de blanka. Försegla sedan plattan med en tätningsfilm innan du lägger den i en inkubator vid 37 grader Celsius i 30 minuter.Efter att ha tvättat plattan ytterligare fem gånger, tillsätt 50 mikroliter av var och en av färgframkallarna, A och B. Skaka plattan försiktigt för att blanda väl. Inkubera sedan plattan vid 37 grader Celsius i svagt ljus i 10 minuter. Pipettera nu 50 mikroliter av stopplösningen i varje brunn för att avsluta reaktionen.Mät absorbansen för varje brunn sekventiellt vid 450 nanometer. Plasmakoncentrationen av homocystein i metioningruppen var två gånger högre än i kontrollgruppen. Plasmakoncentrationerna av homocystein var relativt sett lägre i behandlingsgrupperna.

Research

JoVE Journal

Biochemistry

Plasma Homocysteine (Hcy) ELISA Measurement of Blood from H-Type Hypertensive Rats Treated with Huotan Jiedu Tongluo Decoction

Antihypertensive Effects of Huotan Jiedu Tongluo in H-Type Hypertensive Rats

The Antihypertensive Effects and Mechanisms of Huotan Jiedu Tongluo Decoction in Rats with H-Type Hypertension

Author Spotlight: Exploring Huotan Jiedu Tongluo Decoction as an Antihypertensive Drug

H-type hypertension, which is a specific form of hypertension characterized by elevated plasma homocysteine (Hcy) levels, has become a major public health ...

Treatment of Methionine-Induced H-Type Hypertensive Rats with Huotan Jiedu Tongluo Decoction (HTJDTLD)

Behandling av metionin-inducerade H-typ hypertensiva råttor med Huotan Jiedu Tongluo Avkok (HTJDTLD)