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Quantifying Dendritic Spine Morphology Accurately and Reproducibly Using a Neuron Reconstruction Software

651 Views

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06:50 min

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September 27th, 2024

DOI :

10.3791/201923-v

September 27th, 2024

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Kevin M. Keary III¹, Ellen Sojka¹^,², Melissa Gonzalez¹^,², Zheng Li¹

¹Section on Synapse Development Plasticity, National Institute of Mental Health, National Institutes of Health, ²Colgate University

Transcript

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Dendritic Spine Morphology

Neuron Reconstruction Software

Neurolucida 360

Confocal Images

Hippocampal Slices

Dendrite Tracing

User Guided Tracing

Kernel Population

Precision Tracing

Detection Settings

Detector Sensitivity

Spine Detection

Thickness Modification

From the series

Protocol for Dendritic Spine Analysis Using Neurolucida 360

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