Research
Education
Solutions
Sign In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
TR - Türkçe
JA - 日本語
PL - Polski
RU - Русский
43 Views
•
01:17 min
June 21st, 2024
DOI :
10.3791/201927-v
Transcript
首先,获得一只 6 至 8 周龄的雌性小鼠,每隔 42 至 48 小时注射 PMSG,然后注射 HCG。将雌性小鼠与年龄相仿的雄性小鼠交配。对雌性小鼠实施安乐死后,打开腹部并找到其生殖器。
使用镊子拉伸输卵管、卵巢和脂肪垫。用剪刀剪断输卵管和卵巢,然后剪断子宫。将获得的输卵管转移到预装有 M2 培养基的 35 毫米培养皿中。
穿刺输卵管扩大的壶腹以释放或挤压积云合子复合体。使用镊子将复合物转移到含有
Sign in or start your free trial to access this content
Explore More Videos
From the series
Privacy
Terms of Use
Policies
Contact Us
Recommend to library
JoVE NEWSLETTERS
JoVE Journal
Methods Collections
JoVE Encyclopedia of Experiments
Archive
JoVE Core
JoVE Business
JoVE Science Education
JoVE Lab Manual
Faculty Resource Center
Authors
Librarians
Access
ABOUT JoVE
Copyright © 2025 MyJoVE Corporation. All rights reserved