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33 Views
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02:30 min
June 21st, 2024
DOI :
10.3791/201928-v
Transcript
首先,从新鲜交配的雌性小鼠中获取胚胎。在 35 毫米培养皿的底部制备 12 微滴单纯钾优化培养基。用 3 到 5 毫升矿物油覆盖微滴,并将培养皿放入 37 摄氏度和 5% 二氧化碳的培养箱中以平衡。
使用移液管将小鼠胚胎从 M2 培养基转移到 KSOM 培养基中。将洗涤后的胚胎放入准备好的 KSOM 微滴中,并在 37 摄氏度和 5% 二氧化碳下孵育四天。根据它们的大小、内细胞质量、滋养层细胞的数量和扩增程度选
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