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Please note that all translations are automatically generated. Click here for the English version.
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02:01 min
July 12th, 2024
DOI :
10.3791/201998-v
Transcript
首先,获得注射有预染有CM-Dil的白血病细胞的斑马鱼胚胎。将麻醉的胚胎转移到1.5毫升离心管中,并使用P200移液管,将蛋黄溶解在100微升无钙林格溶液中5分钟。将每个脱黄胚胎样品与一毫升胰蛋白酶胶原酶溶液在 29 摄氏度下孵育 30 至 35 分钟。
使用 P1000 吸头,每五分钟在该溶液中上下移液胚胎,直到骨架结构不再可见。为了停止反应,向试管中加入 200 微升 FBS。充分混合并再孵育 5 分钟。
将细胞悬浮液以 300 G 在 4 摄氏度下沉淀 5 分
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