Sign In

A subscription to JoVE is required to view this content.

A Reverse Genetic Approach to Test Functional Redundancy During Embryogenesis

11.9K Views

06:59 min

August 11th, 2010

August 11th, 2010

11,925 Views

0:07

Title

0:59

Introduction

1:55

Validation of Morpholinos

4:23

Co-Injection of Morpholinos

4:54

Evaluation of Phenotypes

5:37

Representative Reuslts

6:24

Conclusion

Gene function can be obscured in loss-of-function experiments if there is compensation by another gene. The zebrafish model provides a relatively high-throughput means to reveal such functional redundancy in living embryos.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More