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19 Views
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02:22 min
February 23rd, 2024
DOI :
10.3791/202021-v
* These authors contributed equally
Transcript
首先,在 NGM 琼脂平板上转移 500 至 1, 000 个 L1 阶段同步秀丽隐杆线虫,并在 20 摄氏度下孵育直至测试当天。为两个实验组设计检测计划。使用 M9 缓冲液,在相应日期收集 L4 蠕虫和成年蠕虫。
将收集的蠕虫转移到 1.5 毫升微管中,等待蠕虫在重力作用下沉淀。用 1 毫升 M9 缓冲液洗涤蠕虫 3 次,最后一次洗涤后在试管中留下大约 500 微升。使用自动移液器,将 10 微升蠕虫悬浮液添
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评估秀丽隐杆线虫的代谢和衰老模式
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