establishment of the rat ileal pouch model using microsurgical techniques

<meta charset="utf8"></head><body>Eksperimentel rottemodel af pouchitis til undersøgelse af ulcerøs colitis patogenese og behandling

Pouchitis is a non-specific inflammation that affects the ileal pouch and is a prevalent complication following total proctocolectomy and ileal pouch-anal anastomosis (IPAA) in individuals with ulcerative colitis (UC)1,2,3. This condition has a relatively high occurrence rate of up to 50% and can cause various clinical manifestations, including diarrhea, abdominal pain, fecal blood loss, and fever. The exact cause of pouchitis remains elusive, although some researchers believe that a shift in the pouch flora may trigger immune activation and subsequent inflammation4,5,6,7.
Due to the challenges associated with conducting clinical trials on pouchitis, animal models can serve as valuable tools for studying pouchitis drugs and mechanisms. There are growing concerns regarding the creation of rat ileal pouches, with reports indicating possible inflammation8. However, research in this field remains sparse due to the intricate nature of the manufacturing process, which lacks clear guidelines9,10. In 1998, Lichtman was the first to establish an ileal pouch model in Lewis rats and Sprague-Dawley (SD) rats by performing total colectomy11. They observed macrophage infiltration, mucosal ulceration, and an increase in anaerobic bacterial flora within the intestines of these rats, providing a solid foundation for further research on ileal pouch inflammation. This experimental model of rat pouchitis closely mimics the physical signs and underlying mechanisms observed in human pouchitis.
Commonly applied preclinical ulcerative colitis models include the DSS and TNBS models. The inducing chemical 2,4,6-trinitrobenzene sulfonic acid (TNBS) typically simulates Crohn&#39;s disease12. The DSS model, respected for its efficacy, safety profile, and affordability, is often used as a reliable tool for UC induction due to the evident symptoms observed. Given the colonization of the pouch tissue, we successfully induced a pouchitis model using DSS13,14.
In the present study, microsurgery was used to successfully create an ileal pouch model in experimental rats via IPAA surgery. Subsequently, a sustained rat pouchitis model was established by inducing inflammation of the ileal pouch with DSS. Accuracy during surgery is essential for successful model formation, and postoperative care is crucial as well. This model can be used to investigate the pathogenesis of pouchitis, evaluate potential therapeutic agents, and further our understanding of this complex condition. The study streamlines the ileal pouch manufacturing procedure, reducing operation duration and boosting efficiency, thereby establishing a robust foundation for fundamental research into postsurgical pouch disorders.

<meta charset="utf8"></head><body>Forfatter Spotlight: Udvikling af en rottemodel til forskning og behandling af puuchitis

En rottemodel af pouuchitis efter proktokolektomi og ileal pose-anal anastomose ved hjælp af dextransulfatnatrium

Due to the challenges associated with conducting clinical trials on pouchitis, animal models can serve as valuable tools for studying pouchitis drugs and their mechanisms. This protocol describes the method for establishing a rat model of pouchitis. This experimental animal model of pouchitis provides a foundation for studying the pathogenesis and the treatment of the condition.With previous experience in mouse feeding and IPAA surgery modeling, compressing the learning curve down to around 10 subjects is feasible. A critical step in this process is construction and anastomosis of the J-pouch. Postoperative management also play a pivotal role in successful modeling.The successful establishment of the rat pouchitis model provide a solid platform for subsequent drug and mechanism research. Our team will always be committed to studying the pathogenesis of pouchitis and the mechanism of therapeutic drugs.

Etablering af Rat Ileal Pouch-modellen ved hjælp af mikrokirurgiske teknikker

establishment-rat-ileal-pouch-model-using-microsurgical

Research

JoVE Journal

Medicine

Establishment of the Rat Ileal Pouch Model Using Microsurgical Techniques

A Rat Model of Pouchitis Following Proctocolectomy and Ileal Pouch-Anal Anastomosis Using Dextran Sulfate Sodium

Ulcerative colitis (UC) is a chronic immune-mediated disease that affects the entire colon and rectum with a relapsing and remitting course, causing lifelong morbidity. When medical treatment is ineffective, especially in cases of massive gastrointestinal bleeding, perforation, toxic megacolon, or carcinogenesis, surgery becomes the last line of defense to cure UC. Total colorectal resection and ileal pouch-anal anastomosis (IPAA) offer the best chance for long-term treatment. Pouchitis is the most common and troublesome postoperative complication. In this investigation, microsurgery is employed to create an ileal pouch model in experimental rats via IPAA surgery. Subsequently, a sustained rat model of pouchitis is established by inducing inflammation of the ileal pouch with dextran sulfate sodium (DSS). The successful establishment of rat pouchitis is validated through analysis of postoperative general status, weight, food and water intake, fecal data, as well as pouch tissue pathology, immunohistochemistry, and inflammatory factor analysis. This experimental animal model of pouchitis provides a foundation for studying the pathogenesis and treatment of the condition.

This protocol describes the method for establishing a rat model of pouchitis. The ileal pouch model was created by performing ileal pouch-anal anastomosis (IPAA) surgery using microsurgical techniques. After the surgery, the rat was treated with 4% dextran sulfate sodium (DSS) for 4 days.

Ulcerative colitis (UC) is a chronic immune-mediated disease that affects the entire colon and rectum with a relapsing and remitting course, causing ...

To begin, position the rat on its back in dorsal recumbency on an anatomical bench. Cover the surgical site with sterile gauze soaked in bacteriostatic saline. After creating a six-centimeter midline incision, reveal the peritoneal surface using a retractor.Then, cut the terminal jejunum. Position the cecal stump one to two centimeters from the ileocecal valve and then cut it. Isolate the blood supply to the terminal jejunum and tie it off with an 8-0 suture thread.Now, begin the resection of the right hemicolon, carefully tying off the right hemicolon and middle colic vein. Next, separate the inferior mesenteric artery and isolate the inferior rectal artery. Proceed to isolate down the rectum until reaching two centimeters from the anal verge.Then, cut the distal rectum at an angle of approximately 45 degrees to prevent postoperative narrowing. Then, fold the terminal ileum into a J shape to create a pouch for the ileum. Using an interlocking stitch, perform posterior wall anastomosis and enhance the anterior wall with a modified Connell stitch.Ensure there is no torsion in the mesentery. At intervals, suture the anterior side walls of the pouch's opening and the lateral wall of the rectal stump using an 8-0 suturing thread for traction. Then, apply a full layer continuous lock suture to both the anterior and posterior walls.Sequentially close the abdominal muscle fascia and skin using 4-0 sutures. To treat the rat ileal pouchitis model with DSS, dissolve four grams of DSS in 100 milliliters of pure water, freshly prepared daily. Administer pure water to the IPAA group and 4%DSS to the pouchitis group from postoperative day 31 to day 35.Initially, both the IPAA and ileal pouchitis groups had equal body weights and were generally in good condition. By the 35th day after surgery, the weight of the ileal pouchitis group was significantly lower than that of the IPAA group.