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02:30 min
July 26th, 2024
DOI :
10.3791/202036-v
Transcript
为了从分离的神经元脑中获得单细胞悬液,将大脑置于预冷的 15 毫升玻璃 Dounce 匀浆器中,该匀浆含有适量冰冷的 Hanks 平衡盐溶液或 HBSS 缓冲液。使用松散的 Dounce 杵在冰上轻轻缓慢地敲击约 100 至 120 次,使脑组织分离。通过 50 毫升离心管中的 70 微米细胞过滤器过滤所得脑组织悬液。
用 5 毫升 HBSS 冲洗 Dounce 管并继续过滤以最大限度地收集细胞。将过滤的组织悬液在 4 摄氏度下以 550 g 离心 6 分钟。使用真空抽吸器去除
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