high-resolution cell transplantation in zebrafish for nerve development studies

<meta charset="utf8"></head><body>Rejenerasyon ve Gelişim Araştırmaları için Sağlam Zebra Balığı Hücre Nakli

Cell transplantation has a long and storied history as a foundational technique in developmental biology. Around the turn of the 20th century, approaches using physical manipulations to perturb the developmental process, including transplantation, transformed embryology from an observational science into an experimental one1,2. In one landmark experiment, Hans Spemann and Hilde Mangold ectopically transplanted the dorsal blastopore lip of a salamander embryo onto the opposite side of a host embryo, inducing the nearby tissue to form a secondary body axis3. This experiment showed that cells could induce other cells to adopt certain fates, and subsequently, transplantation developed as a powerful method for interrogating critical questions in developmental biology regarding competence and cell fate determination, cell lineage, inductive ability, plasticity, and stem cell potency1,4,5.
More recent scientific advances have expanded the power of the transplantation approach. In 1969, Nicole Le Douarin&#39;s discovery that nucleolar staining could distinguish species of origin in quail-chick chimeras allowed for the tracking of transplanted cells and their progeny6. This concept was later supercharged by the advent of transgenic fluorescent markers and advanced imaging techniques5, and has been leveraged to track cell fate6,7, identify stem cells and their potency8,9, and track cell movements during brain development10. Additionally, the rise of molecular genetics facilitated transplantation between hosts and donors of distinct genotypes, supporting precise dissection of autonomous and non-autonomous functions of developmental factors11.
Transplantation has also made important contributions to the study of regeneration, particularly in organisms with strong regenerative abilities such as planarians and axolotl, by elucidating the cellular identities and interactions that regulate the growth and patterning of regenerating tissues. Transplant studies have revealed principles of potency12, spatial patterning13,14, contributions of specific tissues15,16, and roles for cellular memory12,17 in regeneration.
Zebrafish are a leading vertebrate model for the study of development and regeneration, including in the nervous system, due to their conserved genetic programs, high genetic tractability, external fertilization, large clutch size, and optical clarity18,19,20. Zebrafish are also highly amenable to transplantation at early developmental stages. The most prominent approach is the transplantation of cells from a labeled donor embryo to a host embryo at the blastula or gastrula stage to generate mosaic animals. Cells transplanted during the blastula stage will scatter and disperse as epiboly begins, producing a mosaic of labeled cells and tissues across the embryo21. Gastrula transplants allow for some targeting of transplanted cells according to a rough fate map as the shield forms and the A-P and D-V axes can be determined21. The resulting mosaics have been valuable in determining whether genes act cell autonomously, testing cell commitment, and mapping tissue movement and cell migration throughout development5,11. Mosaic zebrafish can be generated in several ways, including electroporation22, recombination23, and F0 transgenesis24 and mutagenesis25, but transplantation provides the greatest manipulability and precision in space, time, and number and types of cells. The current state of zebrafish transplantation is largely constrained to progenitor cells at early stages, with a few exceptions including transplantation of spinal motor neurons26,27, retinal ganglion cells28,29, and neural crest cells in the first 10-30 h post fertilization (hpf)30, and of hematopoietic and tumor cells in adult zebrafish5,31. Expanding transplantation methods to a broad range of ages, differentiation stages, and cell types would greatly enhance the power of this approach to provide insights into developmental and regenerative processes.
Here, we demonstrate a flexible and robust technique for high resolution cell transplantation effective in zebrafish embryos and larvae up to at least 7 days post fertilization. Transgenic host and donor fish expressing fluorescent proteins in target tissues can be used to extract single cells and transplant them with near-perfect spatial and temporal resolution. The optical clarity of zebrafish embryos and larvae allows for the transplanted cells to be imaged live as the host animal develops or regenerates. This approach has previously been used to examine how spatiotemporal signaling dynamics influence neuronal identity and axon guidance in the embryo32, and to examine the logic by which intrinsic and extrinsic factors promote axon guidance during regeneration in larval fish33. While we focus here on the transplantation of differentiated neurons, our method is widely applicable to both undifferentiated and differentiated cell types across many stages and tissues to address questions in development and regeneration.

<meta charset="utf8"></head><body>Yazar Spotlight: Gelişim ve Rejenerasyon Sırasında Zebra Balığı Sinir Sisteminde Hücresel Bağlantının Haritalanması

Embriyonik ve Larva Zebra Balıklarında Yüksek Çözünürlüklü Hücre Nakli

Our lab studies how cellular connectivity is patterned in the nervous system during development and regeneration. We examine how environmental signals influence neural behavior by using cell transplantation to address how their behavior changes when they're placed in different genetic spatial or temporal environments. While transplantation is common in zebrafish embryos, it's generally performed during the blastula and gastrula stages when cells are largely undifferentiated.This is valuable for generating genetic mosaics, but it limits the ability of the technique to interrogate how spatial temporal signaling dynamics influence development beyond these stages. Here, we present a protocol that allows for transplantation of cells, including differentiated neurons in embryos and larva up to at least seven days post fertilization. We can transplant as little as one cell with high spatial and temporal resolution and use genetic fluorescent labeling for long-term tracking of transplanted cells in the host animal.This protocol allows us to alter the genetic spatial and temporal environment of cells at many different developmental stages, opening up new opportunities to examine principles of style signaling during development and regeneration.

Sinir Gelişimi Çalışmaları için Zebra Balıklarında Yüksek Çözünürlüklü Hücre Nakli

high-resolution-cell-transplantation-zebrafish-for-nerve-development

Research

JoVE Journal

Developmental Biology

High-Resolution Cell Transplantation in Zebrafish for Nerve Development Studies

62 Views. To begin, insert a micropipette into the micropipette puller and pull a microinjection needle. Align the needle tip with the divisions of the stage micrometer under a dissecting scope. With the micrometer as a measurement guide, use a sharpened pair of forceps to break the needle to a bore size slightly larger than the cell's diameter.Using a 50-milliliter syringe equipped with a pipette filler, completely fill the needle with the mineral oil. On the transplant apparatus, turn the thre...