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Flow Cytometry of Hoechst-Stained Mouse Bone Marrow Cells for Effective Detection of Side Population Using High-Power 375 nm and 405 nm Lasers

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04:11 min

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August 23rd, 2024

DOI :

10.3791/202210-v

August 23rd, 2024

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1Institute of Hematology, Department of Hematology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, 2The Lab of Aging Research, State Key Laboratory of Biotherapy, National Clinical Research Center for Geriatrics, West China Hospital, Sichuan University, 3Precision Medicine Research Laboratory, West China Hospital, Sichuan University

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Flow Cytometry
Hoechst 33342
Mouse Bone Marrow
Side Population
High power Lasers
QC Standardization
Experiment Setup
Gain Adjustment
Gate Settings
Live Cell Detection
Fluorescent Signals
Dye Stimulation
SP Cells
Two dimensional Plots

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Alternative Laser Excitation Method for Easy Side Population Cell Detection
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