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Please note that all translations are automatically generated. Click here for the English version.
119 Views
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01:16 min
April 19th, 2024
DOI :
10.3791/202267-v
* These authors contributed equally
Transcript
首先,称取 50 克花生蠕虫的重量,将它们与 150 毫升盐水一起加入均质器中。以 24, 000 RPM 的转速均质化 60 秒。离心匀浆并收集上清液用于进一步处理。
对于纤维蛋白原聚丙烯酰胺凝胶制备,将 0.01 克纤维蛋白原和其他试剂加入 50 毫升玻璃烧杯中。将烧杯在 37 摄氏度下加热 30 分钟。加入丙烯酰胺后,将分离凝胶混合物倒入 10 孔凝胶模具中。
然后在凝胶模具中加入 2 毫升双
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使用 Fibrinogen-PAGE 揭示花生蠕虫中的纤维蛋白原溶解剂
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