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01:16 min
July 26th, 2024
DOI :
10.3791/202312-v
* These authors contributed equally
Transcript
首先用每毫升 5 毫升 10 微克的聚-L-鸟氨酸包被 T25 培养瓶,然后孵育。为了将大脑与小鼠胚胎分离,用组织镊子提起安乐死的怀孕小鼠下腹部的皮肤。使用解剖剪刀,从该点到下肋骨做一个 V 形切口。
用组织镊子抓住含有胚胎的子宫角,轻轻地将胚胎从腹腔中取出。使用弹簧剪刀,在颅骨顶部创建一个中线切口,从脖子后部开始到鼻子。将颅骨从切口部位提起,露出大脑。
用组织镊子轻轻舀出大脑,然后将大脑转移到含有
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