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01:56 min
November 1st, 2024
DOI :
10.3791/202400-v
Transcript
首先,在设置为 23 摄氏度的受控环境室内,在潮湿的土壤中种植 Nicotiana benthamiana 种子,保持 16 小时的光照,然后是 8 小时的黑暗。将两周大的幼苗转移到更大的花盆中后,在相同条件下种植植物四到五周,为农分泌实验做准备。将农杆菌划线到含有所需载体的 Mephasins EHA105 细胞上到 LB 琼脂平板上,并在 28 摄氏度下孵育。
两天后,将单个菌落从平板转移到 2 毫升 LB
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