Deparaffinization and Rehydration of Tissue Sections: A Two-step Procedure to Remove Paraffin Wax from FFPE Tissue Slides Followed by Tissue Rehydration

To begin, prepare 10 micrometers of unstained formalin-fixed paraffin-embedded sections. Place the slides in a glass slide holder and fill it with xylene, making sure that all tissue on the slide is submerged. Leave the slides in xylene for 15 minutes. Then, pour out the xylene while holding the slides with a pipette tip so that they do not fall out. Pour in more xylene to the same level as before and repeat the incubation.

Pour out the xylene and fill the slide holder with 100% ethanol, making sure that all the tissue on the slide is fully submerged. Leave the slides in the ethanol for 3 minutes. Then, replace the ethanol with fresh ethanol and allow them to incubate for two more minutes. Pour off the ethanol and remove the slides. Carefully place them face up on a clean paper towel and allow them to dry for 10 minutes.