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JoVE Journal

Medicine

This content is Open Access.

Assessment of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy

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11:38 min

August 31st, 2010

August 31st, 2010

25,971 Views

0:00

Title

0:59

Introduction

1:25

Experimental Set-up

4:33

Isolated Heart Preparation

7:41

Assessment of Cardiac Function and Energetics Using 31P NMR Spectroscopy

9:43

Representative Results of Cardiac Function and Energetics in Isolated Mouse Hearts Using 31P NMR Spectroscopy

11:09

Conclusion

Langendorff-mode isolated heart perfusion, in conjunction with 31P NMR spectroscopy, combines the fields of biochemistry and physiology into one experiment. The protocol allows for the dynamic measurement of high energy phosphate content and turnover in the heart while concurrently monitoring physiologic function. When performed correctly, this is a valuable technique in the assessment of cardiac energetics.

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