Exertional Heat Stroke Mouse Model: A Protocol to Study Mechanisms Underlying Exertional Heat Stroke

Exertional heat stroke or EHS is caused by a rapid increase in body temperature due to physical overexertion in hot and humid conditions, leading to neurological dysfunction and multi-organ damage.

To establish a preclinical model for EHS, take a mouse implanted with a radiotelemetry device that allows continuous monitoring of the core body temperature. Place the mouse in an artificial climate chamber equipped with a forced running wheel and a temperature monitoring device.

Set the temperature to ambient levels for acclimatizing the mouse to the chamber. Once the mouse is stable, record its body weight. Next, set the temperature and humidity of the chamber to target levels. Simultaneously, surround the chamber with a black-out curtain to reduce light disturbance.

Once the chamber has reached its target conditions, quickly open the chamber door and place the mouse on the forced running wheel. Begin moving the wheel and gradually increase the speed. The mouse's body temperature rises as a result of the exertion. After the mouse has reached its maximum core temperature, keep the wheel speed constant.

Watch the mouse for signs of exhaustion to stop the wheel. Subsequently, remove the mouse from the artificial climate chamber and weigh it. Shift the mouse to its home cage and allow the animal to recover at ambient temperature.