Fluorescence-Detection Size-Exclusion Chromatography: A Technique to Identify the Integrity of Fluorescent Membrane Proteins upon Detergent Solubilization

For fluorescence-detected size-exclusion chromatography or FSEC analysis, next, equilibrate a size-exclusion column with a broad fractionation range with running flow buffer at a rate of 0.3 milliliters per minute. Then, inject 100 microliters of a solubilized membrane sample onto the column at the same flow rate, and monitor the illusion profile using fluorescence optics.